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ppsB [2018-02-13T09:04:11.000Z]
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ppsB [2018-02-13T09:04:11.000Z]

plipastatin synthetase
locus
BSU18330
pI
5.06
mw
289.84 kDa
protein length
2560 aa Sequence Blast
gene length
7680 bp Sequence Blast
function
production of the antibacterial compound plipastatin
product
plipastatin synthetase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.1|Biosynthesis of antibacterial compounds]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.15|Biosynthesis of antibacterial compounds]

    • Gene

    Coordinates
    1,982,565 → 1,990,247

    The protein

    Protein family

  • ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

    • Paralogous protein(s)

  • shares highly conserved domains of more than 1,000 amino acids with: [protein|95B75CFF126AF0AD845E62B4036DD3B6DAC8C7FA|DhbF], [protein|13EB6B903088C11D985DDB7CEC864797058FE038|PksJ], [protein|66AFF1FC7D1BBA8EA5BB57A935FF5AB890A895C1|PksN], [protein|E3D7EF8F165CAC2682B0D620081C5C6E555E43C4|PpsA], [protein|E7A2F93B33B1899313893D362D92405232BC9097|PpsB], [protein|D11CE89E2AC2ADE3FEFEC261E295EC5906CE6407|PpsC], [protein|E73D9F1A8F54DAD4B2056CABBE580D7AD36CB042|PpsD], [protein|C1C051209943E02369356075EAA733297E9478CB|PpsE], [protein|81845F44CE2C601555066E31E87384FF5D7B4139|SrfAA], [protein|3C484FDB3A440269E87D6B6E8500A875AC456666|SrfAB], [protein|1D5F227860A321506A1AB4BAE63CAD3A66E43BFC|SrfAC]

    • Structure

  • [PDB|2VSQ] (termination module of [protein|1D5F227860A321506A1AB4BAE63CAD3A66E43BFC|SrfAC], 38% identity) [pubmed|18583577]

    • Expression and Regulation

    Operons

    genes
    [gene|E3D7EF8F165CAC2682B0D620081C5C6E555E43C4|ppsA]-[gene|E7A2F93B33B1899313893D362D92405232BC9097|ppsB]-[gene|D11CE89E2AC2ADE3FEFEC261E295EC5906CE6407|ppsC]-[gene|E73D9F1A8F54DAD4B2056CABBE580D7AD36CB042|ppsD]-[gene|C1C051209943E02369356075EAA733297E9478CB|ppsE]
    description
    [Pubmed|20817675]

    regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
    • view in new tab

    Biological materials

    Mutant

  • BKE18330 (Δ[gene|E7A2F93B33B1899313893D362D92405232BC9097|ppsB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAATTCCACACACCTTT, downstream forward: _UP4_AAACTATAGGAGAGGAGATT
  • BKK18330 (Δ[gene|E7A2F93B33B1899313893D362D92405232BC9097|ppsB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAATTCCACACACCTTT, downstream forward: _UP4_AAACTATAGGAGAGGAGATT

    • References

  • 10471562,9387222,20817675,18583577